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Mold
Test Kit Counting Practice
Practice Tutorial on
Mold Colony Counting
Basic
Terms:
For the MoldCheck™ Mold
Test Kit Films:
Mold Spores are too small to be seen. It generally takes
three to four days for a mold spore to grow into a mold colony large enough to be seen.
A Mold Colony first appears as a pencil point
dot on the MoldCheck™
film.
This is a single colony which has grown from a single spore which settled onto
the film. The spore is digesting the food nutrients on the film and growing
"vine like" structures called hyphae. The mold colony will
continue to grow with a generally circular shape, "fuzzy" edges, a "bulls eye" center, and a variety of colors,
such as blue, black, orange, pink and green. Mold colonies produce mold spores ("seeds") which grow like grape clusters at the end of the fuzzy "hairs" called
hyphae. Each colony can eventually produce thousands of spores if allowed
to grow free.
Yeast will first appear on the film as small pencil point sized or slightly larger dot with blue to blue-green to off white color without a "bulls eye" center.
Overlapping Colonies
Mold colonies tend to grow rapidly after four days and will begin to overlap each other as well as cover the yeast. Two or three colonies will often butt-up against each other.
A double cluster will look generally like this:

A triple cluster will look generally like this:

Color Wash-Over
Yeast - if the film has turned all one bluish or bluish-green color, it may be the result of too many yeast
colonies to count.
Mold - if the film is quickly covered in black, blue, yellow, orange, dots, and
colors, it may have too many mold colonies to count.
When this color wash-over or excessive density growth occurs, the area must be retested for a shorter period of time. Reduce the sampling time to ½ the original time and try again.
Practice Counting:
Count the mold and yeast on these
MoldCheck™
films and see if you get the same number (click
here for answers). The yeast are shown in
blue.

Practice Count 1
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| ___ |
Mold |
___ |
Yeast (blue) |
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Practice Count 2
| |
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| ___ |
Mold |
___ |
Yeast (blue) |
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Practice Count 3
| |
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| ___ |
Mold |
___ |
Yeast (blue) |
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Practice Count 4
| |
|
|
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| ___ |
Mold |
___ |
Yeast (blue) |
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Practice Count 5
| |
|
|
|
| ___ |
Mold |
___ |
Yeast (blue) |
| |
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| If
there are many mold colonies on a film, it is acceptable to count 1/4
of the film and multiply by four (4) to obtain your total mold
spore count. |
|
NOTE: Even professionals will disagree on some
counts by a few colonies! |